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Download PDF, EPUB, MOBI Papers from the Tortugas Laboratory of the Carnegie Institution of Washington Volume 3
Download PDF, EPUB, MOBI Papers from the Tortugas Laboratory of the Carnegie Institution of Washington Volume 3

Papers from the Tortugas Laboratory of the Carnegie Institution of Washington Volume 3. Carnegie Institution Laboratory

Papers from the Tortugas Laboratory of the Carnegie Institution of Washington Volume 3
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Author: Carnegie Institution Laboratory
Number of Pages: 54 pages
Published Date: 01 Mar 2012
Publisher: Rarebooksclub.com
Publication Country: Miami Fl, United States
Language: English
ISBN: 9781130905021
File size: 12 Mb
File Name: Papers.from.the.Tortugas.Laboratory.of.the.Carnegie.Institution.of.Washington.Volume.3.pdf
Download Link: Papers from the Tortugas Laboratory of the Carnegie Institution of Washington Volume 3
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This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1911 Excerpt: ...excellent work of Miiller on the green turtle, supplemented by Fritsch's work on the land turtle, and by such dissections as the writer has made on the mud turtles found in the vicinity of Iowa City. The specimens dissected were mostly Chrysemys marginata, although specimens of ChelyJra serpentina and of Amyda mutica were also studied. The turtles were killed with chloral hydrate or with chloroform. The blood-vessels were injected with red and with blue gelatin, while the lymphatics were injected with a thin yellow gelatin mass. The melting-point of the gelatin was lowered by adding potassium iodid to the melted solution. The posterior lymph hearts were studied by exposing them after the animal had been chloroformed. As they continue to beat for some time after they have been exposed, they are very easily found and studied. Their connections with adjacent veins can readily be demonstrated by injecting India ink into their cavity. For the preparation of the embryonic material a large number of fixing agents were used. Corrosive sublimate solutions were, as a rule, not satisfactory, although Zenker's fluid gave fairly good results. Gilson's fluid proved very unsatisfactory, especially with large embryos. The mesenchymal tissues were poorly fixed and the material did not keep well. By far the best results for embryos of all stages were obtained by using mixtures of chromic and acetic acids with pure formaldehyde. These mixtures seem to penetrate the thick shell more readily and to fix the internal organs more thoroughly and with less distortion than any other combination tried. The following mixture for embryos up to about 30 days of development was found satisfactory: Chrom-aceto-Formaldehyde:1 Chromic acid, 1 per cent aq. solution, 64 parts; glacial acetic a...

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